Elisa faranti ga Elisa Assayi Kit

Gyarawa: ƙara rijiyoyin mara kyau na 3 mara kyau, 1 na kyakkyawar iko, da 100ul na Merum.
Sanya: Elisa Elisa 96 Ana buƙatar girgiza don sanya samfurin cikakke gauraye, sannan kuma an sanya shi a cikin 37 ° C in in in in in incubator ko ruwa mai aiki na mintina 20.
Wanke farantin Elisa: tsarma da mafita mafi kyau 15m tare da distilled ruwa zuwa girma 300ml. Lokacin da wanke hannu, abubuwan da ke ciki a cikin rijiyoyin farantin da ya kamata a zubar da shi, ya kamata a cika maganin wankewar da rijiyar dauki, sannan kuma an sanya shi tsawon mintuna 30 sannan kuma ya girgiza sosai. Maimaita wannan aikin don sau 5 sannan kuma fara zuwa bushe bushe. Wanke na inji yana buƙatar aiwatar da shi sau 5, kowane rami ne allura tare da 200u na mafita na wanke ko za'a iya cika shi, sannan tsaya
Bayan minti 30, magudana da kuma bushe.
Addara Enzyme Yi wajan magance mafita na aiki: kara 100ul na enzyme lakabin mafita ga kowane rijiya, ka kuma wanke shi da aka yi amfani da shi sau 20 bayan mintina 20. Aikin wanke Elisa Micriya daidai yake da matakai na sama.
Wannan shine matakin farko na amsawar, wanda shine ci gaba mai launi da dakatar da amsawar: 50ul na substrate an kara wa da minti 10 a cikin duhu a karkashin yanayi 37. Addara tsattsauka ga kowane rijiya
An gauraya 50ul don dakatar da abin.
Yin amfani da farawar kayan kwalliyar bunsen microtiter yana buƙatar aiwatar da shi a ƙarƙashin yanayin bakararre. Dignuwa da tsabtatawa yakamata a yi a hankali bayan amfani da farantin microtiter, amma ya kamata a lura cewa wasu enzymes
Kafin notin kamuwa da daidaitattun kwamitin, kuna buƙatar karanta umarnin a hankali don ganin ko ana iya haifuwa a cikin tsananin zazzabi.